Great Experiments: How do growing nerve cells find their targets?

How do growing nerve cells find their targets?

Credit: Nobel Foundation

In 1949, Rita Levi-Montalcini noticed something unexpected. Her colleague Elmer Bueker had implanted tumors into chick embryos and found that nerves would invade the tumors, but what attracted nerves to tumors? Levi-Montalcini had noticed that the nerves were not just invading tumors: They were also invading the tissues near the tumors, even invading blood vessels downstream of tumors — suggesting that the tumors might have been releasing a diffusible nerve growth factor (NGF), a postulated substance that could guide either nerve differentiation, growth or survival. Levi-Montalcini proved the existence of NGF by culturing just tumors and ganglia in the same dish, finding that the nerves from the ganglia would connect to tumors even in vitro. Later, she, Stanley Cohen, and colleagues purified NGF. NGF told us that the way nerves find even their normal targets is unexpectedly adaptive: nerves grow just about everywhere, and they die off where they fail to find targets.

The paper: Levi-Montalcini, R. (1952) Effects of mouse tumor transplantation on the nervous system. Ann N Y Acad Sci. 55:330-44.

A short review: Aloe, L. (2004) Rita Levi-Montalcini: the discovery of nerve growth factor and modern neurobiology. Trends Cell Biol 14:395-9.

Some amazing historical background: An excerpt about her pre-NGF work, which she did in a makeshift home lab that she set up hiding out from Nazis and fascists during WWII, from her autobiography, In Praise of Imperfection. Open the excerpt in Acrobat and you'll see some helpful notes in red.

No comments:

Post a Comment

Note: Only a member of this blog may post a comment.

FOR COURSE PARTICIPANTS:

Our goal: to learn about important, beautifully designed experiments from across the biological and biomedical sciences.

In the course, you'll gain experience in reading classic papers and explaining key experiments from your own fields of interest to colleagues in other fields.

Students propose papers to discuss in the form of short written posts on this site. Papers to discuss are selected by class vote each week.

This course should strengthen your communication skills and your understanding of diverse fields of biology–and importantly, it should inspire creative thinking about your own research.

Writing your posts

Each person should contribute at least 4 posts about their favorite experiments by 1 month before the end of the semester (by the end of October). Please propose your own schedule for 4 posts in an email to Bob.

Please post about experiments that you consider to be (1) fundamental to an area of science and (2) beautifully designed. Experiments that changed or founded a field and also made you hit your head and say, "damn, why didn't I think of that?!" are great choices.

Posts should be 150 words or fewer, plus a picture or movie with an appropriate image credit included. Please do not exceed 150 words. This is an exercise in clear, concise writing.

Your post should be written for an intelligent audience who understands biology but knows little to nothing about the field you're writing about. Avoid confusing jargon. Informal wording is fine. Your post's title should capture what's interesting and fundamental about the work, for example the central question addressed by the work.

Please provide a link to the one paper you chose, bullet-pointed so that there's no confusion about which one this is. If you like, below the one bullet-pointed link you can also provide a link to a companion paper or two that can help put the main paper into context.

It's considered very bad form to cut and paste text from programs like Microsoft Word for your post, because hidden formatting comes along and can cause headaches. It makes me sad. A tip: copying your text from Word into a Google search bar, and then copying that text from the search bar, eliminates the formatting.

You can modify your posts at any time. It's okay to rewrite your dusty old posts to try to re-invigorate interest.

Voting

Voting will take place each Friday until 5pm on a poll that will be sent to you. Each poll will generally include all posts that are up by that Friday at 8am.

Please vote every week. Please don't vote for your own selections. The voting will decide which paper we'll read for the following week's meeting. Posts that are getting few or no votes will be removed periodically.

Meetings

We'll meet each Thursday at 12:30pm in GSB3101. Each week, we'll discuss the winning paper. The person who chose it will present it along with anything else that seems relevant, with everyone's help, tolerating constant interruptions for some interesting discussion.

An important part of the course: Everyone else should plan to present one other thing briefly and informally to everyone–a related paper, some historical background, or anything at all you chose to explore because you found it interesting and relevant. Come prepared to answer questions, since we're all curious.

If you don't have time to do the readings one week, please skip the meeting that week. This way, everyone who comes will be full participants. No one will be badgered for missing just 1 or 2 meetings, I swear.

-Bob Goldstein